QD introduces the Quantum C100, born from discussions in the Cryo-EM field about what makes ideal detector performance at 100keV. The Quantum C100, combined with specially optimised 100 keV microscopes, is set to transform the field of Cryo-EM by enabling top quality publishable data whilst operating at a much lower energy. This detector aims to allow the radical simplification of the microscope design and make single particle analysis more affordable and widely-used across science and industry.
This new generation of detector technology is being developed by the Science and Technology Facilities Council (STFC), in partnership with the Rosalind Franklin Institute (RFI) and MRC Laboratory of Molecular Biology (MRC LMB).
Roger Goldsbrough, CEO of Quantum Detectors, said:
“We’ve been providing advanced detector solutions for almost 15 years now, and the introduction of the Quantum C100 will take product innovation to another level. We are absolutely delighted to have been selected to partner with the Rosalind Franklin Institute and the STFC, to help bring CryoEM technology to a far wider scientific community, with a whole new class of instrumentation delivering the higher quality data that scientists need.”
What is Cryo-EM?
Transmission electron microscopy (TEM) is used to image the structures of materials and large molecules. In TEM, a beam of electrons is passed through an ultrathin sample, and following interaction with the sample, the electrons are detected, enabling an image of the sample to be constructed in high resolution. Samples analysed by TEM are exposed to intense high-energy electron beams under strong vacuum conditions, which can lead to the degradation of some samples, in particular biomolecules. Cryo-EM (cryogenic electron microscopy) overcomes these issues by imaging samples at low temperatures, allowing the structures of biomolecules, such as proteins and nucleic acids, to be determined in high resolution. Cryo-EM is an attractive alternative to conventional X-ray crystallographic methods, as it images biomolecules in solution and does not require a crystallised sample. The significance of Cryo-EM was recognised internationally in 2017, when the Nobel Prize in Chemistry was awarded to Richard Henderson, Jacques Dubochet and Joachim Frank for their seminal work in the development and applications of this technique.
|Operation Energy||100 keV|
|Sensor Format||2048 x 2048|
|Pixel Pitch||50 x 50 μm|
|Frame Rate||2000 fps 2500 fps|
|Bit Depth||12 bits 10 bits|
|Chip Size||120 mm x 125 mm|
|Active Area||102.4 mm x 102.4 mm|
How is the Quantum C100 different?
Large active area
With excellent DQE and 2K x 2K pixels, the Quantum C100 detector is designed to produce better data from lower energy microscopes than existing detectors working with higher energy microscopes.
Flexible data analysis approach
The Quantum C100 exports data in an open format so is adaptable for your lab, and aims to work with popular plug-ins.
The Quantum C100 will have a user friendly (but powerful) GUI interface to make data-capture quick and straightforward.
Fastest camera for Cryo-EM
The Quantum C100 is a fast detector – at speeds of more than 2000 Hz (continuous mode) it is excellent for minimising radiation damage to the sample.
Mounting to new or existing microscopes
The Quantum C100 can be bottom mounted to existing TEMs, or supplied in combination with new TEMs. Contact us for more details.
Recently published articles supporting 100keV Cryo-EM:
- K Naydenova, G McMullan, MJ Peet, Y Lee, PC Edwards, S Chen, E Leahy, S Scotcher, R Henderson, CJ Russo (2019)
CryoEM at 100 keV: a demonstration and prospects
IUCrJ 6: 1086-1098.
- Peet MJ, Henderson R and Russo CJ (2019)
The energy dependence of contrast and damage in electron cryomicroscopy of biological molecules
Ultramicroscopy 203: 125-131.
Bell DC, Russo CJ, Benner G. (2010)
Microscopy and Microanalysis Volume 16, Iss 4.
A potential difference for single-particle cryo-EM
aStructural Biology of Cells and Viruses Laboratory, The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK
*Correspondence e-mail: firstname.lastname@example.org